@article{El-Nagar_Abd El-Azeem_Nasef_Sultan_2017, title={Prevalence of Toxigenic and Methicillin Resistant Staphylococci in Poultry Chain Production}, volume={7}, url={https://www.advetresearch.com/index.php/AVR/article/view/1}, abstractNote={<p>Staphylococci are a worldwide cause of human and animal infection and are considered to be of the most common causes of infections in birds. Enterotoxins produced by some staphylococcal species were recognized as a causative agent of staphylococcal food poisoning (SFP). Only enterotoxins produced by <em>Staphylococcu</em>s <em>aureus</em> were as yet well characterized. Much less is known about&nbsp;enterotoxigenic&nbsp;potential&nbsp;of&nbsp;coagulase-negative&nbsp;species of genus Staphylococcus (CNS). It has been reported that&nbsp;enterotoxigenic&nbsp;CNS strains have been associated with human and animal infections and food poisoning. Samples collected from chicken production cycle (un hatched eggs, baby chicks, broilers, chicken meat and table eggs) in Luxor, Egypt were tested to investigate the presence of Staphylococcus species and detection of their enterotoxines genes with more special attention for detection of methicillin resistance gene (<em>mec A</em>). Samples were tested for <em>S. aureus</em> and CNS on the basis of cultural and biochemical properties and confirmed by PCR amplification of <em>16S rRNA</em> and <em>clfa </em>gene. Results showed that the presence of Staphylococci were 50/150 (33.3%), 14% of the samples were <em>S. aureus </em>(21/150), while, 19.33% were CNS (29/150).<em> mecA</em> gene was detected in 66.7% and 51.7% among <em>S. aureus</em> and CNS respectively. Enterotoxins genes (<em>seb, sec</em> and <em>see) </em>were found in 5 (23.8%) of <em>S. aureus with a percent of </em>(9.5%) for<em> seb </em>and <em>sec </em>and (4.8%) for <em>see, </em>while <em>sec</em> and <em>see </em>were found in 6 (20.6%) of CNS.&nbsp; With a percent (10.3%) for each. &nbsp;</p>}, number={2}, journal={Journal of Advanced Veterinary Research}, author={El-Nagar, Shimaa and Abd El-Azeem, Mohamed Wael and Nasef, Soad A. and Sultan, Serageldeen}, year={2017}, month={Apr.}, pages={33-38} }