Monitoring of Helicobacter pylori in chicken products

Abstract

A total of 200 samples of chicken products collected randomly from Quesna and El-Bagour in Menoufia governorate. The collected samples were represented by chicken breast, thigh, gizzard and liver (50 of each) and subjected to bacteriological examination for detection of Helicobacter species particularly, Helicobacter pylori. Further, the effect of L. rhamnosus culture to control such serious pathogen contaminating chicken products was studied. Helicobacter spp detected in 4%, 6%, 12% and 20% of breast, thigh, gizzard and liver, respectively and H. pylori represent 4%, 4% 6% and 10% from this examined samples, respectively. H. pylori inoculated chicken fillet (1×10⁶ /g) which are not treated with  Lactobacillus rhamnosus (control +ve) showed increase  in  prevalence  of H. pylori 1.0×10⁶, 1.3×10⁶, 1.9×10⁶ and 2.4×10⁶ at zero time, 1^st day, 2^nd day and 3^rd day, respectively.  While chicken fillets treated with L. rhamnosus (1x10⁶ /g) Effect on prevalence of H. pylori inoculated into chicken fillets with a reduction percent of  35%, 52% and 69%  but  L. rhamnosus (1x10⁷ /g) showed remarkable reduction of H. pylori prevalence  with a reduction percent of  54%, 67% and 81% at 1^st day, 2^nd day and 3^rd day, respectively. L. rhamnosus effected on virulence genes of H. pylori isolated from treated chicken fillet as L. rhamnosus (1x10⁶/g) destructed cagA gene and L. rhamnosus (1x107/g) destructed cagA and vacA genes so using of Lactobacillus rhamnosus culture as a method of control has a good effect on prevalence of H. pylori and its virulence genes in chicken fillets and L. rhamnosus (1x10⁷ /g) more effective than L. rhamnosus (1x10⁶ /g).