Histological and Histochemical Studies on the Ampulla of the Deferent Duct of Donkey (Equus asinus)

Abstract

The present study was performed on the ampulla of the deferent duct of 24 sexually mature apparently healthy male donkeys (5 to 7 years) distributed over the four seasons of the year. The ampullary glands were branched tubulo-alveolar in type which they were opened in a central lumen. The luminal and the glandular epithelium were formed of principal and basal cells. Singly scattered rod- shaped cells were observed among the principal cells. PAS positive reactivity was observed in the surface and the glandular epithelium of the ampulla ductus deferentis. Only the luminal contents and the secretory materials attached to the apical border of the principal cells, showed positive reactivity for alcian blue. The present study presents the first description of the ampulla ductus deferentis of donkey. In addition to, it acts as a reservoir for sperms it had a secretory function for neutral and acid mucopolysaccharid.