Study on the Effect of Adding Bovine Serum Albumin to Semen Diluent on the Viability of Awassi Ram Semen Preserved at 5oC

  • Osama Ibrahim Azawi
  • Elias Khudhur Hussein

Abstract

This study was aimed to test the effect of adding bovine serum albumin (BSA) to Tris-fructose-egg yolk diluent to increase the ram sperm storage period at 5ºC. Semen samples from six mature Awassi rams used in this study. Semen samples were diluted by Tris-glucose-egg yolk. Diluted semen sample was divided into two parts. The first part BSA was added 10 mg ml-1 and the second part considered as a control group without any addition. The diluted semen samples were cooled and preserved at 5oC for 5 days. Cooled diluted semen samples were examined for individual motility, percent of live sperm, sperm abnormalities and acrosomal defects every 24 h until 5 days. Results of the present study showed an increased viability of spermatozoa diluted in the Tris diluent containing BSA stored at 5oC for 120 h of preservation compared with the control group. Spermatozoa motility and viability declined gradually in BSA Tris containing diluent when preserved at 5oC from 0 h to 120 h to 54.0±0.9% and 59.0±0.9 %, respectively compared to control of Tris diluent without BSA which was declined markedly to 35.9±1.9 %, 40.9±1.8 %, respectively. In conclusion, this study demonstrated that supplementation with BSA of ram semen diluents during semen preservation at 5oC, may exert beneficial effects on the quality of the Awassi ram semen.

Published
2011-10-01
How to Cite
AZAWI, Osama Ibrahim; HUSSEIN, Elias Khudhur. Study on the Effect of Adding Bovine Serum Albumin to Semen Diluent on the Viability of Awassi Ram Semen Preserved at 5oC. Journal of Advanced Veterinary Research, [S.l.], v. 1, n. 3, p. 115-118, oct. 2011. ISSN 2090-6277. Available at: <https://www.advetresearch.com/index.php/AVR/article/view/237>. Date accessed: 19 july 2019.
Section
Original Research

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