Assessment of Drinking Water Quality and New Disinfectants for Water Treatment in a Small Commercial Poultry Farm

Abstract

This work designed to monitor the hygienic quality of drinking water supply in a small commercial poultry farm and to assess the efficiency of different new disinfectant compounds against some pathogenic bacterial isolates. A total of 60 water samples was collected from both the main source and drinkers for physico-chemical and bacteriological examination. The sensitivity pattern of 40 strains of bacterial isolates to commonly used disinfectants in poultry facilities for water supply treatment was evaluated using the broth macro-dilution method. Results, the mean values of both alkalinity and total hardness were found in the highest rate of 183.0±17.6 and 345.6±7.6 mg/l compared to their values in the main water source. Furthermore, the mean value of ammonia in drinkers besides nitrite, and phosphate discovered at the highest level compared to the main water supply (1.36±0.31, 3.4±0.46, 26.3±0.78 mg/l, respectively). Both E. coli and Shigella spp. in drinkers were detected at the highest isolation rate (22.6%). Salmonella kentucky (S. kentucky: O20, 8 H I) isolates were found at the highest rate of 57.1%. Whilst the pathogenic E. coli serotyping Poly3 (O157) recorded at 66.67%, followed by E. coli O114 33.33%. Biocidal efficiency of Klorsept 25 disinfectant against investigated pathogenic bacterial isolates was 100% at a concentration of 2.0 mg/l after 180 min of exposure. Whilst the efficiency of calcium hypochlorite Ca (Ocl)2 against E. coli and S. kentucky was 100% at a concentration of 0.5 mg/l and exposure time 120 min. The susceptibility of all bacterial isolates to H2O2 disinfectant at a concentration of 5.0 % was 100% within 60 min contact time. In conclusion, the investigation of hygienic quality of water supply should be occurred periodically to ensure the safety of water source for poultry chick's health. The sensitivity of the studied pathogenic bacterial isolates is 100% to Klorsept 25 disinfectant at a concentration of 2.0 mg/l, calcium hypochlorite (Ca (Ocl)2) at 0.5 mg/l, and H2O2 at 5.0 % at exposure time does not exceed 180 min.