Improving Landrace Pig Sperm Motility and Kinematics by Adding Citrate and Glucose to Coconut Water-Egg Yolk Diluent: A CASA Study

Abstract

Sperm motility and kinematics are considered significant elements that could affect the artificial insemination programs in pigs. The objectives of this experiment were to investigate the impact of citrate and glucose supplementation to coconut water-egg yolk-based diluents on the motility and kinematics of landrace pig sperm. The research material was fresh semen of a landrace pig having total sperm motility greater than 70% and sperm abnormality less than 20%. The semen was diluted with four different diluents: CY (young coconut water-egg yolk, control), CYG (young coconut water-egg yolk with glucose), CYC (young coconut water-egg yolk with citrate), and CYGC (young coconut water-egg yolk with glucose and citrate). Sperm motility and kinematics were assessed at 48 hours of preservation using Computer Assisted Sperm Analysis (CASA), while sperm morphology was assessed under light microscopy. The revealed sperm motility was significantly different (P<0.05) in the experimental treatments. The best result in motility was obtained by CYGC diluent with total motility of 76.86%, progressive motility at 50.35%, and fast motility of 24.76%, while the Sperm abnormalities were no significant difference (P > 0.05). The highest sperm kinematics were also produced by CYGC and were significantly different from CY and CYG for all sperm kinematics parameters (P<0.05), while with the CYC diluent it was significantly different only in several parameters such as DCL, DSL, DAP, BCF, HAC, LIN, and STR (P<0.05). It could be concluded that supplementing the coconut water-egg yolk diluent with glucose and citrate could enhance the motility and kinematics of Landrace pig sperm.