Liquid Semen Quality of Landrace Boar in Tris-Egg Yolk Extender and Zorlesco Extender at 20 °C

Abstract

Liquid semen quality of landrace boar during preservation needs an extender to maintain sperm from the negative effect such as loss of energy, pH change, and membrane permeability damage. To reduce the negative effects, good extenders are required. Tris-EY and zorlesco are two common extenders that used for the preservation of boar semen. This research was aimed to evaluate that ability of tris-EY extender and zorlesco extender to maintain the quality of landrace liquid semen at 20 °C. Semen was collected from 3 years of landrace boar in healthy condition, by hand glove method twice a week. A completely randomized design with 2 treatments and 7 replications were used to collect data. Semen was evaluated macroscopically and microscopically then extended with tris-EY extender and zorlesco extender. The motility and viability of sperm were evaluated every six hours. Data were analyzed by T-test, SPSS Programed. Research results showed that sperm motility of landrace semen in a tris-EY extender and zorlesco extender has no significant difference (P ˃ 0.05) at 24 h evaluation, which were 41.00±2.23% in a tris-EY extender and 45.00±5.00% in zorlesco extenders. The viability of sperm also has no significant difference (P ˃ 0.05), the observed values were 54.38±6.38% in a tris-EY extender and 58.32±10.35% in a zorlesco extender. It can be concluded that tris-EY extender and zorlesco extender have a similar ability in maintaining the quality of landrace boar liquid semen at 20°C.