Prolactin gene in duck based on Genbank data sequences: A preliminary study

Abstract

The prolactin gene is a candidate gene for egg weight, egg number, and egg production in ducks. This study aimed to perform genetics analysis, including single nucleotide polymorphisms, amino acid change, restriction enzyme, and phylogenetic tree. A preliminary study on the prolactin gene was carried out in 8 GenBank sequences of ducks. Polymorphism was screened in 8 prolactin gene sequences of duck (AB158611.1; JQ677091.2; GU984377.1; DQ660983.1; DQ345782.1; LC565023.1; NM001310372; DQ345783.1) using alignment with Bioedit ver. 7.2. The results showed that 87 SNPs were detected: 10 SNPs in the 5’UTR region, 3 SNPs in exon 1, 25 SNPs in intron 1, 4 SNPs in exon 2, 18 SNPs in intron 2,  2 SNPs in exon 3, 8 SNPs in intron 3, 1 SNP in exon 4, 9 SNPs in intron 4; 5 SNPs in exon 5 and 2 SNPs in 3’UTR. There were 9 SNPs in the exon region that changed amino acids. The main result of restriction enzyme mapping was discovered seven enzymes (AluI, BstKTI, DpnI, MboI, AciI, FatI, NlaIII) which recognized 4 SNPs regions based on restriction mapping using Bioedit. The recommendation for a restriction enzyme for the next step was the AluI enzyme. The results of this study provide further evidence of the role of the Prolactin gene as a candidate gene in duck.